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Antibodies

AbFlex® Histone H3K27me3 antibody (rAb)

Catalog No: 91167 Format: 100 µg $375 Buy Now
Catalog No: 91168 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG
Purification:Protein A Chromatography
Host:Rabbit
Molecular Weight:17 kDa
Reactivity:Human

Applications

Western Blot Validated Dot Blot Validated

for AbFlex Histone H3K27me3 antibody (rAb)Application Notes


Validated Applications:
WB: 0.1 - 1 µg/ml
Note: For optimal results, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for detection of Histone H3K27me3.

for AbFlex Histone H3K27me3 antibody (rAb)Immunogen

This Histone H3 trimethyl Lys27 antibody was raised against a peptide containing trimethyl Lys27 of human Histone H3.

for AbFlex Histone H3K27me3 antibody (rAb)Buffer

140mM Hepes, pH 7.5, 70mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.

View our Guide to Histone Modifications and Biological Function.

 
AbFlex<sup>®</sup> Histone H3K27me3 antibody (rAb) tested by Western blot.

AbFlex® Histone H3K27me3 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H3K27me3 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H3K27me3 antibody (rAb) tested by dot blot analysis.

AbFlex® Histone H3K27me3 antibody specificity is shown by Dot Blot.
Recombinant proteins were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K27me1 (MLA), Cat. No. 31621; Lane 3 - Histone H3K27me2 (MLA), Cat. No. 31597; Lane 4 - Histone H3K27me3 (MLA), Cat. No. 31579. Lane 5 - Histone H3K4me1 (MLA), Cat. No. 31619; Lane 6 - Histone H3K4me2 (MLA), Cat. No. 31595; Lane 7- Histone H3K4me3 (MLA), Cat. No. 31600; Lane 8 - Histone H3K9me1 (EPL), Cat. No. 31281; Lane 9 - Histone H3K9me2 (EPL), Cat. No. 31596; Lane 10 - Histone H3K9me3 (EPL), Cat. No. 31601; Top Panel was probed with Histone H3 antibody (mAb) at 2 µg/ml. Bottom Panel was probed with AbFlex Histone H3K27me3 antibody at 2 µg/ml.

for AbFlex Histone H3K27me3 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H3K27me3 antibody was expressed in 293 cells, and contains rabbit immunoglobulin heavy and light chains.

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Lysine 27 of histone H3 can be mono-, di- or trimethylated (Histone H3 monomethyl Lys27, Histone H3 dimethyl Lys27 or Histone H3 trimethyl Lys27) by different histone methyltransferases such as EZH2 or NSD3. Methylation of this residue is mainly associated with transcriptional repression.

for AbFlex Histone H3K27me3 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Histone H3K27me3 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® Histone H3K27me3 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Histone H3K27me3 antibody (rAb) tested by Western blot.

Western blot of AbFlex® Histone H3K27me3 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Histone H3K27me3 antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® Histone H3K27me3 antibody (rAb).
(Click image to enlarge and see details.)