Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
Antibodies

AbFlex® His-Tag antibody (rAb)

Catalog No: 91183 Format: 100 µg $375 Buy Now
Catalog No: 91184 Format: 10 µg $75 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Reactivity:Other (Not Species Specific)

Applications

Western Blot Validated

for AbFlex His-Tag antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/mL

for AbFlex His-Tag antibody (rAb)Immunogen

The His-Tag is comprised of a series of six or more histidine residues that are used to label the carboxy or amino terminus of a recombinant protein. This antibody is designed to recognize the His-Tag (HHHHHH).

for AbFlex His-Tag antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> His-Tag antibody (rAb) tested by Western blot.

AbFlex® His-Tag antibody (rAb) tested by Western blot.
His-Tagged protein (125 ng per lane) probed with AbFlex® His-Tag antibody (1 µg/ml dilution).

for AbFlex His-Tag antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.

AbFlex® His-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The 6xHis-Tag that is normally present on the AbFlex antibody was removed to avoid self binding.

for AbFlex His-Tag antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex His-Tag antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® His-Tag antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> His-Tag antibody (rAb) tested by Western blot.

Western blot of AbFlex® His-Tag antibody (rAb).
(Click image to enlarge and see details.)