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Antibodies

AbFlex® HDAC2 antibody (rAb)

Catalog No: 91197 Format: 100 µg $375 Buy Now
Catalog No: 91198 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:55 kDa
Reactivity:Human

Applications

ChIP Validated ChIP-Seq Validated Western Blot Validated

for AbFlex HDAC2 antibody (rAb)Application Notes


Validated Applications:
ChIP-Seq: 4 - 10 µg per ChIP
WB: 0.2-2 µg/ml

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for AbFlex HDAC2 antibody (rAb)Immunogen

This antibody was raised against peptide corresponding to amino acids 473-488 of human HDAC2.

for AbFlex HDAC2 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.07% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> HDAC2 antibody (rAb) tested by ChIP-Seq.

AbFlex® HDAC2 recombinant antibody (rAb) tested by ChIP-Seq
Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 30 µg of chromatin from NCI-H1437 (human lung carcinoma cell line) chromatin and 4 µg HDAC2 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 15.6 million sequence tags were mapped to identify HDAC2 binding sites on chromosome 1.

AbFlex<sup>®</sup> HDAC2 antibody (rAb) tested by Western blot.

Western blot of AbFlex® HDAC2 antibody (rAb).
20 µg HeLa cell nuclear extract was run on SDS-PAGE and probed with 0.5 µg/ml AbFlex® HDAC2 antibody (rAb).

MW: 55 kDa

for AbFlex HDAC2 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.  HDAC2 (Histone Deacetylase 2, also designated mammalian RPD3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with AbFlex® HDAC2 antibody (rAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use AbFlex® HDAC2 antibody (rAb).

for AbFlex HDAC2 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex HDAC2 antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® HDAC2 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> HDAC2 antibody (rAb) tested by ChIP-Seq.

ChIP-Seq of AbFlex® HDAC2 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> HDAC2 antibody (rAb) tested by Western blot.

Western blot of AbFlex® HDAC2 antibody (rAb).
(Click image to enlarge and see details.)