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Antibodies

AbFlex® HDAC3 antibody (rAb)

Catalog No: 91199 Format: 100 µg $375 Buy Now
Catalog No: 91200 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:52 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex HDAC3 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/mL

NOTE: primary antibody incubations require addition of 0.1% Tween 20 to all Blotto solutions to detect specific bands without background.

for AbFlex HDAC3 antibody (rAb)Immunogen

This antibody was raised against a peptide within the C-terminal region of human HDAC3.

for AbFlex HDAC3 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 39717.

 
AbFlex<sup>®</sup> HDAC3 antibody (rAb) tested by Western blot.

AbFlex® HDAC3 antibody (rAb) tested by Western blot.
20 µg of HeLa cell extract was run on SDS-PAGE and probed with AbFlex HDAC3 antibody at 1 µg/ml.

for AbFlex HDAC3 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® HDAC3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

HDAC3 (Histone Deacetylase 3) is a member of the class I mammalian histone deacetylases (HDACs) involved in regulating chromatin structure during transcription. These enzymes catalyze the removal of acetyl groups from lysine residues of histones and other cellular proteins. Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in regulation of gene expression in various cellular functions. It consists of the transfer of an acetyl moiety from an acetyl coenzyme A to the ε-amino group of a lysine residue.

for AbFlex HDAC3 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex HDAC3 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® HDAC3 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> HDAC3 antibody (rAb) tested by Western blot.

Western blot of AbFlex® HDAC3 antibody (rAb).
(Click image to enlarge and see details.)