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Antibodies

AbFlex® ATM phospho Ser1981 antibody (rAb)

Catalog No: 91207 Format: 100 µg $375 Buy Now
Catalog No: 91208 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:370 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated Dot Blot Validated

for AbFlex ATM phospho Ser1981 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml
DB: 0.5 - 1 µg/ml

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

for AbFlex ATM phospho Ser1981 antibody (rAb)Immunogen

This antibody was raised against A peptide surrounding phosph-serine 1981 of human ATM (Ataxia telangiectasia mutated).

for AbFlex ATM phospho Ser1981 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> ATM phospho Ser1981 antibody (rAb)  tested by Western blot.

AbFlex® ATM phospho Ser1981 antibody (rAb) tested by Western blot.
20 µg HeLa nuclear extract (high salt preparation) untreated (lane 1) or treated with VP16 at 100 µM for 8 hrs (Lane 2), probed with 1 µg/ml ATM phospho Ser1981 antibody.
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

AbFlex<sup>®</sup> ATM phospho Ser1981 antibody (rAb)  tested by dot blot analysis.

AbFlex® ATM phospho Ser1981 antibody specifity
Dot blot analysis was used to confirm the specificity of ATM phospho Ser1981 antibody. Single-stranded DNA oligonucleotides (amount of oligo in nanograms listed on the left side of the blot) were spotted on to a positively charged nylon membrane and blotted with antibody (0.5 µg/ml dilution).
1: ATM phospho serine 1981 (SLAFEEG(Sph)QSTTISS)
2: H2AX phospho serine 139 (CKATQA[Sph]QEY)
3: ATM unmodified serine 1981 (SLAFEEGSQSTTISS)
4: NBS phospho serine 343 (CPGPSL[Sph]QGVS)
5: BRCA1 phospho serine 1423 (LEQHG[Sph]QSNSC)

for AbFlex ATM phospho Ser1981 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.  ATM – Ataxia Telangiectasia Mutated is a PI3-family protein kinase and a critical cell cycle checkpoint protein. ATM and a related protein, ATR, play crucial roles in the maintenance of genome integrity and the response to DNA damage. Upon activation, ATM phosphorylates itself at serine 1981 and then phosphorylates a number of downstream proteins (e.g. BRCA1, CHK2, H2AX, p53, NBS1, SMC1) resulting in cell cycle arrest and the initiation of DNA damage repair. Loss of function of ATM is causal to a variety of syndromes involving increased incidence of several cancers.

for AbFlex ATM phospho Ser1981 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex ATM phospho Ser1981 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® ATM phospho Ser1981 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> ATM phospho Ser1981 antibody (rAb)  tested by Western blot.

Western blot of AbFlex® ATM phospho Ser1981 antibody (rAb) .
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> ATM phospho Ser1981 antibody (rAb)  tested by dot blot analysis.

Dot blot of AbFlex® ATM phospho Ser1981 antibody (rAb) .
(Click image to enlarge and see details.)