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Antibodies

AbFlex® c-Myc antibody (rAb)

Catalog No: 91213 Format: 100 µg $375 Buy Now
Catalog No: 91214 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:68 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex c-Myc antibody (rAb)Application Notes


Validated Applications:
WB: 1 µg/ml dilution.

for AbFlex c-Myc antibody (rAb)Immunogen

This antibody was raised against amino acids 1-262 of human c-Myc (myc proto-oncogene protein).

for AbFlex c-Myc antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035% sodium azide, 30% glycerol

 
AbFlex<sup>®</sup> c-Myc antibody (rAb) tested by Western blot.

AbFlex® c-Myc antibody (rAb) tested by Western Blot.
HEK293 cells were transiently transfected with empty vector (Lane 1) or with human c-Myc protein (Lane 2). 10 µg HEK293 whole cell extract was loaded on SDS-PAGE gel and probed with AbFlex c-Myc antibody at 1 µg/ml. MW: 68 kDa.

for AbFlex c-Myc antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports.  c-Myc is a sequence specific DNA binding transcription factor that regulates gene expression, especially genes involved in cell division. Myc family members contain a basic Helix-Loop-Helix leucine zipper (bHLHZ) domain. Myc family proteins associated with thousands of genes, especially those involved in cell growth and proliferation. Myc serves as an integrator of signal transduction, linking extracellular signals to an acute cellular growth response. Mutations and overexpression of c-Myc are associated with cell proliferation and cancer.

for AbFlex c-Myc antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex c-Myc antibody (rAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® c-Myc antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> c-Myc antibody (rAb) tested by Western blot.

Western blot of AbFlex® c-Myc antibody (rAb).
(Click image to enlarge and see details.)