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Antibodies

AbFlex® SMARCA1 / SNF2L1 antibody (rAb)

Catalog No: 91225 Format: 100 µg $375 Buy Now
Catalog No: 91226 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:130 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/mL

Note: For optimal results, we recommend the addition of 0.1% Tween 20
to all Blotto solutions to reduce background. Individual optimization may
be required.

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 61465.

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Immunogen

This antibody was raised against a recombinant protein encompassing amino acids 571 to 772 of human SNF2L (sucrose nonfermenting 2-like protein 1).

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)References

 
AbFlex<sup>®</sup> SMARCA1 / SNF2L1 antibody (rAb) tested by Western blot.

AbFlex® SMARCA1 / SNF2L1 antibody (rAb) tested by Western blot.
30 µg of HeLa cell nuclear extract (Lane 1) or 20 µg of U87 glioblastoma cell nuclear extract (Lane 2) was run on SDS-PAGE and probed with AbFlex® SMARCA1 / SNF2L1 antibody at 2 µg/ml.

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6xHis-Tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex® SMARCA1/SNF2L1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

SMARCA1 / SNF2L1 (Sucrose Nonfermenting 2-Like Protein 1) is an energy-transducing component of NURF (nucleosome-remodeling factor) and CERF (CECR2-containing-remodeling factor) complexes. Both complexes facilitate the perturbation of chromatin structure in an ATP-dependent manner. SMARCA1 / SNF2L1 is the mammalian ortholog of the Drosophila nucleosome-remodeling ATPase ISWI.

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex SMARCA1 / SNF2L1 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® SMARCA1 / SNF2L1 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> SMARCA1 / SNF2L1 antibody (rAb) tested by Western blot.

Western blot of AbFlex® SMARCA1 / SNF2L1 antibody (rAb).
(Click image to enlarge and see details.)