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Antibodies

AbFlex® Pim2 antibody (rAb)

Catalog No: 91239 Format: 100 µg $375 Buy Now
Catalog No: 91240 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:37 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex Pim2 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/mL

Note: For optimal results, we recommend the addition of 0.1% Tween 20
to all Blotto solutions to reduce background. Individual optimization may
be required.

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones.  For details on the parental clone, see Catalog No. 61159.

for AbFlex Pim2 antibody (rAb)Immunogen

This antibody was raised against a recombinant protein corresponding to human PIM2 (pim-2 oncogene).

for AbFlex Pim2 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Pim2 antibody (rAb) tested by Western blot.

AbFlex® Pim2 antibody (rAb) tested by Western blot.
20 µg of K562 cytoplasmic extract was run on SDS-PAGE and probed with AbFlex Pim2 antibody at 0.5 µg/ml. This antibody recognizes all three isoforms of human Pim2.

for AbFlex Pim2 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Pim2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. The antibody recognizes all three isoforms of human Pim2.

PIM2 is a serine/threonine protein kinase in the CAMK family. PIM2 promotes cell survival in response to a variety of proliferative signals through activation of the I-κB kinase/NF-κB pathway. It also inhibits apoptosis through the phosphorylation of BAD. PIM2 has been implicated in the transformation of both T and B lymphocytes and is highly expressed in human leukemia and lymphomas, as well as adenocarcinomas and hepatocellular carcinomas.

for AbFlex Pim2 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Pim2 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Pim2 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Pim2 antibody (rAb) tested by Western blot.

Western blot of AbFlex® Pim2 antibody (rAb).
(Click image to enlarge and see details.)