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Antibodies

AbFlex® Dicer antibody (rAb)

Catalog No: 91241 Format: 100 µg $375 Buy Now
Catalog No: 91242 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:240 kDa
Reactivity:Mouse

Applications

Western Blot Validated

for AbFlex Dicer antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39817.

for AbFlex Dicer antibody (rAb)Immunogen

This antibody was raised against amino acids 1638-1899 of mouse Dicer (aka dicer 1, ribonuclease type III).

for AbFlex Dicer antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Dicer antibody (rAb) tested by Western blot.

AbFlex® Dicer antibody (rAb) tested by Western blot.
40 µg of mouse embryonic stem cell extract was run on SDS-PAGE and probed with AbFlex Dicer antibody at 2 µg/ml.

for AbFlex Dicer antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Dicer antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Dicer is a double-stranded RNase and part of the micro-RNA (miRNA) loading complex (miRLC), which is composed of Dicer, Ago2 and TARBP2.Dicer is required for formation of the RNA induced silencing complex (RISC). Dicer and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto Ago2. Dicer also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the destruction of complementary RNAs.

MicroRNA-3´UTR Interaction Products

If you are interested in this antibody, you may also have an interest in products for studying the functional impact of miRNA-3´UTR interactions. The LightSwitch™ 3´UTR Reporter GoClone™ Collection includes over 12,000 human 3´UTRs that have been cloned into the pLightSwitch_3UTR vector for use in luciferase reporter assays. These constructs can be co-transfected with our collections of LightSwitch miRNA Mimics and Inhibitors to study the effects of over-expression of a miRNA of interest, or to knock down the expression of endogenous human miRNAs in living cells.

for AbFlex Dicer antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Dicer antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Dicer antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Dicer antibody (rAb) tested by Western blot.

Western blot of AbFlex® Dicer antibody (rAb).
(Click image to enlarge and see details.)