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AbFlex® Snail antibody (rAb)

Catalog No: 91255 Format: 100 µg $375 Buy Now
Catalog No: 91256 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Purification:Protein A Chromatography
Molecular Weight:32 kDa


Western Blot Validated

for AbFlex Snail antibody (rAb)Application Notes

Validated Applications:
WB: 0.5 - 2 µg/ml dilution.

Note: For optimal results, we recommend the addition of 0.1% Tween 20
to all Blotto solutions to reduce background. Individual optimization may
be required.

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

for AbFlex Snail antibody (rAb)Immunogen

This antibody was raised against full length recombinant human SNAI1 protein (snail homolog 1 (Drosophila))

for AbFlex Snail antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

AbFlex<sup>®</sup> Snail antibody (rAb) tested by Western blot.

AbFlex® Snail antibody (rAb) tested by Western blot.
20 µg of K562 cell nuclear extract was run on SDS-PAGE and probed with AbFlex Snail antibody at 0.5 µg/ml.

for AbFlex Snail antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Snail antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Snail (snail homolog 1 (Drosophila)) is involved in the epithelial to mesenchymal transition (EMT) and formation and maintenance of embryonic mesoderm (By similarity). Binds to 3 E-boxes of the E-cadherin gene promoter and represses its transcription.

for AbFlex Snail antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Snail antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting

Technical Data Sheet

AbFlex® Snail antibody (rAb)

Data Thumbnails

AbFlex<sup>®</sup> Snail antibody (rAb) tested by Western blot.

Western blot of AbFlex® Snail antibody (rAb).
(Click image to enlarge and see details.)