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Antibodies

AbFlex® Ubiquitin antibody (rAb)

Catalog No: 91257 Format: 100 µg $375 Buy Now
Catalog No: 91258 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:N/A
Reactivity:Other (Wide Range)

Applications

Western Blot Validated

for AbFlex Ubiquitin antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml
Dot Blot: 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39741.

for AbFlex Ubiquitin antibody (rAb)Immunogen

This antibody was raised against bovine ubiquitin.

for AbFlex Ubiquitin antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Ubiquitin antibody (rAb) tested by Western blot.

AbFlex® Ubiquitin antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear acid-extract was run on SDS-PAGE and probed with AbFlex Ubiquitin antibody at 0.5 µg/ml. Antibodies to Ubiquitin will detect proteins of various sizes that are modified by the addition of Ubiquitin.

AbFlex<sup>®</sup> Ubiquitin antibody (rAb) tested by dot blot analysis.

AbFlex®l Ubiquitin antibody specifity
Dot blot analysis was used to confirm the specificity of Ubiquitin antibody. 50 ng, 10 ng, and 2 ng of either recombinant Ubiquitin (Lane 1) or BSA (Lane 2) were spotted onto nylon membrane and probed with AbFlex Ubiquitin recombinant antibody at 2 µg/ml.

for AbFlex Ubiquitin antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Ubiquitin antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Ubiquitin is an important signaling protein that is catalytically added to proteins on lysine residues to modify their function. Addition of multiple ubiquitin proteins in a string (poly-ubiquitylation) to proteins signals their destruction by the proteasome. Mono-ubiquitylation of proteins has a much different effect, more in keeping with post-translational modification of proteins by phosphorylation or acetylation. Mono-ubiquitylation is part of a number of signal transduction pathways, including those involving histone proteins and transcriptional regulation.

for AbFlex Ubiquitin antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Ubiquitin antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Ubiquitin antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Ubiquitin antibody (rAb) tested by Western blot.

Western blot of AbFlex® Ubiquitin antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Ubiquitin antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® Ubiquitin antibody (rAb).
(Click image to enlarge and see details.)