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Antibodies

AbFlex® MEIS 1/2/3 antibody (rAb) 

Catalog No: 91259 Format: 100 µg $375 Buy Now
Catalog No: 91260 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:54-66 kDa
Reactivity:Human, Mouse

Applications

Western Blot Validated

for AbFlex MEIS 1/2/3 antibody (rAb) Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution.

Note: For optimal results, we recommend the addition of 0.1% Tween 20
to all Blotto solutions to reduce background. Individual optimization may
be required.

Application Testing: Parental Monoclonal vs. Recombinant
View PDF file

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39795.

for AbFlex MEIS 1/2/3 antibody (rAb) Immunogen

This antibody was raised against recombinant protein comprising amino acids 59-390 of mouse MEIS1.

for AbFlex MEIS 1/2/3 antibody (rAb) Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> MEIS 1/2/3 antibody (rAb)  tested by Western blot.

AbFlex® MEIS 1/2/3 antibody (rAb) tested by Western blot.
25 µg of SY-SY5Y human neuroblast cell extract was run on SDS-PAGE and probed with AbFlex MEIS 1/2/3 antibody at 2 µg/ml. The antibody recognizes all isoforms of MEIS1.

for AbFlex MEIS 1/2/3 antibody (rAb) Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex MEIS 1/2/3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. This antibody recognizes all isoforms of MEIS1, as determined by Western blot.

The MEIS family or proteins (Myeloid Ecotropic viral Integration Site homologues) encode proteins that function as cofactors for the Homeobox (HOX) proteins. Homeobox genes play a crucial role in normal development. In addition, several homeoproteins are involved in neoplasia. MEIS and a functionally related protein Pbx belong to the TALE (Three Amino acid Loop Extension) homeodomain superfamily of proteins. The role of MEIS proteins in the regulation of transcription is as yet unclear.

for AbFlex MEIS 1/2/3 antibody (rAb) Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex MEIS 1/2/3 antibody (rAb) Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® MEIS 1/2/3 antibody (rAb) 
 

Data Thumbnails

AbFlex<sup>®</sup> MEIS 1/2/3 antibody (rAb)  tested by Western blot.

Western blot of AbFlex® MEIS 1/2/3 antibody (rAb) .
(Click image to enlarge and see details.)