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Antibodies

AbFlex® Histone H3K4me0 antibody (rAb)

Catalog No: 91267 Format: 100 µg $375 Buy Now
Catalog No: 91268 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Reactivity:Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

for AbFlex Histone H3K4me0 antibody (rAb)Application Notes


Validated Applications:
WB: 0.1 – 1 µg/ml
Dot Blot: 0.5 - 1 µg/ml

for AbFlex Histone H3K4me0 antibody (rAb)Immunogen

This antibody was raised against a peptide containing the N-terminus of human histone H3.

for AbFlex Histone H3K4me0 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

View our Guide to Histone Modifications and Biological Function.

for AbFlex Histone H3K4me0 antibody (rAb)References

 
AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by Western blot.

AbFlex® Histone H3K4me0 antibody (rAb) tested by Western blot.
20 µg of HeLa cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by dot blot analysis.

AbFlex® Histone H3K4me0 antibody specificity is shown by Dot Blot.
Recombinant proteins were spotted onto PVDF as follows: Lane 1 - Histone H3 (C110A), Cat. No. 31207; Lane 2 - Histone H3K4me1 (EPL), Cat. No. 31287; Lane 3- Histone H3K4me2 (EPL), Cat. No. 31277; Lane 4 - Histone H3K4me3 (EPL), Cat. No. 31278. Top panel was probed with AbFlex Histone H3K4me0 antibody at 0.5 µg/ml. Bottom panel was probed with Histone H3 mAb (Clone 1B1-B2), Cat. No. 61475, at 1 µg/ml.

for AbFlex Histone H3K4me0 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex H3K4me0 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes. This antibody recognizes the Histone H3, lysine 4, which is unmethylated.

for AbFlex Histone H3K4me0 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Histone H3K4me0 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Histone H3K4me0 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by Western blot.

Western blot of AbFlex® Histone H3K4me0 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> Histone H3K4me0 antibody (rAb) tested by dot blot analysis.

Dot blot of AbFlex® Histone H3K4me0 antibody (rAb).
(Click image to enlarge and see details.)