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Antibodies

AbFlex® BrdU antibody (rAb)

Catalog No: 91269 Format: 100 µg $375 Buy Now
Catalog No: 91270 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:N/A
Reactivity:Other (Wide Range)

Applications

Immunofluorescence Validated

for AbFlex BrdU antibody (rAb)Application Notes


Validated Applications:
Immunofluorescence (IF): 0.5 - 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61273.

for AbFlex BrdU antibody (rAb)Immunogen

This antibody was raised against Bromodeoxyuridine conjugated to BSA (Bovine Serum Albumin).

for AbFlex BrdU antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> BrdU antibody (rAb) tested by immunofluorescence.

AbFlex® BrdU antibody (rAb) tested by Immunofluorescence
HeLa cells were stained with 0.5 µg/ml BrdU antibody, followed by anti-mouse IgG2a – Alexa Fluor 488 (lower panel) or DAPI (upper panel). Prior to staining, HeLa cells were treated for 1 hr at 37°C in cell culture medium supplemented with 10 µM BrdU. DNA was denatured wtih NaOH treatment for detection of BrdU incorporation in cells undergoing DNA replication at the time of labeling.

for AbFlex BrdU antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex BrdU antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

BrdU (Bromodeoxyuridine) is a thymidine analogue, capable of being incorporated into DNA in place of thymidine during DNA synthesis. By pulse labelling with BrdU, the DNA synthesis in the cells can be analysed by detection with anti-BrdU antibodies. Antibodies against BrdU recognize BrdU in single-stranded DNA (produced by partial denaturation of double-stranded DNA), as well as free BrdU or BrdU coupled to a protein carrier.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with AbFlex® BrdU antibody (rAb).

for AbFlex BrdU antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex BrdU antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® BrdU antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> BrdU antibody (rAb) tested by immunofluorescence.

Immunofluorescence stain of AbFlex® BrdU antibody (rAb).
(Click image to enlarge and see details.)