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Antibodies

AbFlex® HA-Tag antibody (rAb)

Catalog No: 91271 Format: 100 µg $375 Buy Now
Catalog No: 91272 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Reactivity:Other (Not Species Specific)

Applications

Immunoprecipitation Validated

for AbFlex HA-Tag antibody (rAb)Application Notes


Validated Applications:
IP: 2 - 5 µg per IP

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39627.

for AbFlex HA-Tag antibody (rAb)Immunogen

This antibody was raised against a peptide containing multiple repeats of the HA tag epitope YPYDVPDYA.

for AbFlex HA-Tag antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> HA-Tag antibody (rAb) tested by immunoprecipitation.

AbFlex® HA-Tag antibody (rAb) tested by Immunoprecipitation.
Whole-cell lysate (400 µg) from U2OS cells expressing an HA-Tagged protein, was immunoprecipitated with 2 µg of AbFlex HA-Tag rAb (lane 1) or control IgG2a (lane 2). Immunoprecipitates were run on SDS-PAGE and analyzed by Western blot using HA-Tag mAb Cat. No. 39627 at 4 µg/ml.

for AbFlex HA-Tag antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex HA-Tag antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use AbFlex® HA-Tag antibody (rAb).

for AbFlex HA-Tag antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex HA-Tag antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® HA-Tag antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> HA-Tag antibody (rAb) tested by immunoprecipitation.

Immunoprecipitation of AbFlex® HA-Tag antibody (rAb).
(Click image to enlarge and see details.)