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Antibodies

AbFlex® CHD3 antibody (rAb)

Catalog No: 91273 Format: 100 µg $375 Buy Now
Catalog No: 91274 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:103 kDa
Reactivity:Drosophila

Applications

Western Blot Validated Immunoprecipitation Validated

for AbFlex CHD3 antibody (rAb)Application Notes


Validated Applications:
Immunoprecipitation (IP): 4 µg per IP
WB: 0.5 - 2 µg/mL

For optimal results, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61469.

for AbFlex CHD3 antibody (rAb)Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acid residues 865-885 of Drosophila.

for AbFlex CHD3 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> CHD3 antibody (rAb) tested by Western blot.

AbFlex® CHD3 antibody (rAb) tested by Western blot.
15 µg of Drosophila Schneider Cell nuclear extract was run on SDS-PAGE and probed with AbFlex CHD3 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> CHD3 antibody (rAb) tested by immunoprecipitation.

AbFlex CHD3 antibody (rAb) tested antibody tested by immunoprecipitation.
Drosophila Schneider cell nuclear extract was immunoprecipitated using 4 µg of AbFlex CHD3 antibody (Lane 1) or an unrelated AbFlex antibody (Lane 2). Protein from IP was probed by Western blot using AbFlex CHD3 antibody (2 µg/ml).

for AbFlex CHD3 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex CHD3 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

CHD3 (chromodomain helicase DNA binding protein 3) is a component of the CHD family of ATP-dependent chromatin remodelers and forms histone deacetylase NuRD complexes which participate in the remodeling of chromatin by deacetylating histones.

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use AbFlex® CHD3 antibody (rAb).

for AbFlex CHD3 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex CHD3 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® CHD3 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> CHD3 antibody (rAb) tested by Western blot.

Western blot of AbFlex® CHD3 antibody (rAb).
(Click image to enlarge and see details.)

AbFlex<sup>®</sup> CHD3 antibody (rAb) tested by immunoprecipitation.

Immunoprecipitation of AbFlex® CHD3 antibody (rAb).
(Click image to enlarge and see details.)