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Antibodies

AbFlex® Mi-2 beta (CHD4) antibody (rAb)

Catalog No: 91275 Format: 100 µg $375 Buy Now
Catalog No: 91276 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:240 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml.

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 39695.

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Immunogen

This antibody was raised against a synthetic peptide derived from human Mi-2 beta.

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Mi-2 beta (CHD4) antibody (rAb) tested by Western blot.

AbFlex® Mi-2 beta (CHD4) antibody (rAb) tested by Western blot
20 µg of Jurkat cell nuclear extract was run on SDS-PAGE and probed with AbFlex Mi-2 beta (CHD4) antibody at 0.5 µg/ml.

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Mi-2beta (CHD4) antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Mi-2 beta (CHD4) antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Mi-2 beta (CHD4) antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Mi-2 beta (CHD4) antibody (rAb) tested by Western blot.

Western blot of AbFlex® Mi-2 beta (CHD4) antibody (rAb).
(Click image to enlarge and see details.)