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Antibodies

AbFlex® MPP8 antibody (rAb)

Catalog No: 91277 Format: 100 µg $375 Buy Now
Catalog No: 91278 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:120 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex MPP8 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml

for AbFlex MPP8 antibody (rAb)Immunogen

This antibody was raised against amino acids 50-120 of human MPP8 (M-phase phosphoprotein 8).

for AbFlex MPP8 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> MPP8 antibody (rAb) tested by Western blot.

AbFlex® MPP8 antibody (rAb) tested by Western blot.
20 µg of HEK293 nuclear extract (Cat. No. 36033) was run on SDS-PAGE and probed with AbFlex MMP8 antibody at 0.5 µg/ml.

for AbFlex MPP8 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex MPP8 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

MPP8 (M-Phase Phosphoprotein 8) was originally identified as a phosphoprotein that reacts with MPM2, an antibody that labels mitosis-specific and cell cycle-regulated phosphoproteins. MPP8 contains a single chromodomain that preferentially interacts with nucleosomes enriched for histone H3 lysine 9 methylation but not lysine 4 methylation.

for AbFlex MPP8 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex MPP8 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® MPP8 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> MPP8 antibody (rAb) tested by Western blot.

Western blot of AbFlex® MPP8 antibody (rAb).
(Click image to enlarge and see details.)