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Antibodies

AbFlex® SaCas9 antibody (rAb)

Catalog No: 91279 Format: 100 µg $375 Buy Now
Catalog No: 91280 Format: 10 µg $75 Buy Now

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Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:130 kDa
Reactivity:Other (Not Species Specific)

Applications

Western Blot Validated

for AbFlex SaCas9 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61787.

for AbFlex SaCas9 antibody (rAb)Immunogen

This antibody was raised against a recombinant protein fragment containing amino acids 1-462 of Staphylococcus aureus Cas9.

for AbFlex SaCas9 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> SaCas9 antibody (rAb) tested by Western blot.

AbFlex® SaCas9 antibody (rAb) tested by Western blot.
20 µg of HeLa whole-cell extract (Lane 1) and HeLa whole-cell extract with 10 ng of full length SaCas9 protein (Lane 2), were run on SDS-PAGE and probed with AbFlex SaCas9 antibody at 0.5 µg/ml.

for AbFlex SaCas9 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex SaCas9 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

SaCas9 is a nuclease from Staphylococcus aureus that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome.

for AbFlex SaCas9 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex SaCas9 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® SaCas9 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> SaCas9 antibody (rAb) tested by Western blot.

Western blot of AbFlex® SaCas9 antibody (rAb).
(Click image to enlarge and see details.)