Active Motif,
Tools to analyze nuclear function,
Your CartYour Cart 0 items
    
Antibodies

AbFlex® Bcl6 antibody (rAb)

Catalog No: 91287 Format: 100 µg $375 Buy Now
Catalog No: 91288 Format: 10 µg $75 Buy Now

Request a quote for a bulk orderBulk Order Button


Antibody Type:Recombinant
Isotype:IgG2a
Purification:Protein A Chromatography
Host:Mouse
Molecular Weight:80 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex Bcl6 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml

AbFlex® recombinant antibodies are genetically derived from DNA sequences of parental hybridoma clones. For details on the parental clone, see Catalog No. 61193.

for AbFlex Bcl6 antibody (rAb)Immunogen

This antibody was raised against full length human Bcl6 (B-cell lymphoma 6 protein) by genetic immunization. (Garcia et. al., 2006. Genetic Immunization: A New Monoclonal Antibody for the Detection of BCL-6 Protein in Paraffin Sections. J. Histochemistry & Cytochemistry, vol. 54(1): 31-38).

for AbFlex Bcl6 antibody (rAb)Buffer

140 mM Hepes, pH 7.5, 70 mM NaCl, 32mM NaOAc, 0.035% sodium azide, and 30% glycerol.

 
AbFlex<sup>®</sup> Bcl6 antibody (rAb) tested by Western blot.

AbFlex® Bcl6 antibody (rAb) tested by Western blot.
20 µg of U87 cell nuclear extract was run on SDS-PAGE and probed with AbFlex Histone Bcl6 antibody at 0.5 µg/ml.

for AbFlex Bcl6 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid upports. AbFlex Bcl6 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells.

Bcl6 (B-cell lymphoma 6 protein, Bcl5, LAZ3, ZBTB27, ZNF51) is sequence-specific transcriptional repressor that is required for the formation of lymph node germinal centers (GC). Bcl6 is a member of the BTB-POZ family of transcriptional regulators and contains six Kruppel-type zinc fingers. The POZ domain in the amino terminus of Bcl6 is involved in the recruitment of corepressor proteins including NCoR, BCoR, SMRT, Mi-2/NURD and several HDACs. Bcl6 promotes proliferative expansion of B-cells in the germinal center through repression of genes regulating cell cycle and differentiation. Bcl6 also suppresses the DNA damage response, to prevent class-switch recombination being repaired. Unregulated expression of Bcl6 is involved in the development of many B-cell lymphomas.

for AbFlex Bcl6 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Bcl6 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Bcl6 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Bcl6 antibody (rAb) tested by Western blot.

Western blot of AbFlex® Bcl6 antibody (rAb).
(Click image to enlarge and see details.)