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Antibodies

AbFlex® Histone H4K20me1 antibody (rAb)

Catalog No: 91291 Format: 100 µg $375 Buy Now
Catalog No: 91292 Format: 10 µg $75 Buy Now

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Isotype:IgG2a
Host:Mouse
Molecular Weight:12 kDa
Reactivity:Human

Applications

Western Blot Validated

for AbFlex Histone H4K20me1 antibody (rAb)Application Notes


Validated Applications:
WB: 0.5 – 2 µg/ml

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. In addition, we recommend the addition of 0.05% Tween 20 to all blocking solutions to reduce background. Individual optimization may be required.

for AbFlex Histone H4K20me1 antibody (rAb)Immunogen

This antibody was raised against a peptide containing monomethyl-lysine 20 of human Histone H4.

for AbFlex Histone H4K20me1 antibody (rAb)Buffer

Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 32 mM NaOAc, 0.035% sodium azide, 30% glycerol.

 
AbFlex<sup>®</sup> Histone H4K20me1 antibody tested by Western blot.

AbFlex® Histone H4K20me1 antibody tested by Western blot.
20 µg of HeLa nuclear extract* was run on SDS-PAGE and probed with AbFlex Histone H4K20me1 antibody at 0.5 µg/ml.

AbFlex<sup>®</sup> Histone H4K20me1 antibody specificity tested by peptide array analysis

AbFlex® Histone H4K20me1 antibody specificity tested by peptide array analysis.
Peptide array analysis was used to confirm the specificity of this antibody for its intended modification. Histone H4K20me1 antibody was applied at 0.1 µg/ml to Active Motif's MODified™ Histone Peptide Array (Catalog No. 13001). The arrays were scanned with ArrayAnalysis Software 7 and the results plotted. Specificity data is shown for the most reactive peptides and those related to the immunogen. View array data here:
Array Data File

for AbFlex Histone H4K20me1 antibody (rAb)Background

AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. Each AbFlex antibody contains a 6XHis tag, an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA, and a sortase recognition motif (LPXTG) to attach a variety of labels directly to the antibody including fluorophores, enzymatic substrates (HRP, AP), peptides, drugs as well as solid supports. AbFlex Histone H4K20me1 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in CHO cells.

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/ exit points.

for AbFlex Histone H4K20me1 antibody (rAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for AbFlex Histone H4K20me1 antibody (rAb)Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting
 

Technical Data Sheet

AbFlex® Histone H4K20me1 antibody (rAb)
 

Data Thumbnails

AbFlex<sup>®</sup> Histone H4K20me1 antibody tested by Western blot.

AbFlex® Histone H4K20me1 antibody tested by Western blot.
(Click to enlarge and view details)

AbFlex<sup>®</sup> Histone H4K20me1 antibody specificity tested by peptide array analysis

AbFlex® Histone H4K20me1 antibody specificity tested by peptide array analysis.
(Click to enlarge and view details)