pLightSwitch_LR

Base pairs                                              3846
Multiple cloning region                                 1-72
Minimal TK promoter					73-272
RenSP reporter gene (synthetic renilla luciferase;
 includes mODC PEST)                                    302-1360
SV40 late poly(A) region                                1391-1612 
ColE1-derived plasmid replication origin                1937
Synthetic Beta-lactamase (Ampr) coding region           2728-3588
Synthetic poly(A) signal/transcriptional pause site     3693-3846
Forward Sequencing Primer: TCCATCAAAACAAAACGAAACAA
Reverse Sequencing Primer: AGTCGAGCACGTTCATCTGCTT

GGCCTAACTGGCCGGTACCTGAGCTCTTACGCGTGCTAGCCCGGGCTCGAGATCTGCGAT
CTAAGTAAGCTTCGTTTGCTGGCGGTGTCCCCGGAAGAAATATATTTGCATGTCTTTAGT
TCTATGATGACACAAACCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATG
CAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGCGTGGCCTCG
AACACCGAGCGACCCTGCAGCGACCCGCTTAAGGCAATCCGGTACTGTTGGTAAAGCCAC
CATGGCTTCCAAGGTGTACGACCCGGAGCAGCGCAAGAGGATGATCACCGGCCCTCAGTG
GTGGGCTCGGTGCAAGCAGATGAACGTGCTCGACTCCTTCATCAACTACTACGACAGCGA
GAAACATGCGGAGAACGCCGTGATCTTCCTCCACGGCAACGCCGCTTCCTCCTACCTGTG
GCGCCACGTCGTGCCCCACATCGAGCCCGTCGCCCGGTGCATCATCCCTGATCTGATCGG
GATGGGGAAGAGCGGGAAGAGCGGCAACGGCAGCTACCGCCTGCTCGACCACTACAAGTA
CCTCACCGCCTGGTTCGAGCTGCTGAACCTCCCCAAGAAGATCATCTTTGTGGGCCACGA
CTGGGGCGCTTGTCTCGCTTTTCACTACTCCTACGAGCACCAGGATAAGATCAAGGCTAT
CGTGCATGCTGAGAGCGTCGTGGACGTGATCGAGTCCTGGGACGAGTGGCCCGATATCGA
GGAGGATATTGCTCTGATCAAGTCCGAGGAGGGCGAGAAGATGGTCCTGGAGAATAACTT
CTTCGTGGAGACTATGCTGCCTAGCAAGATCATGCGCAAGCTGGAGCCCGAGGAGTTCGC
TGCTTACCTGGAGCCCTTCAAGGAGAAGGGCGAGGTCAGAAGACCAACCCTCAGCTGGCC
TCGGGAGATCCCTCTGGTCAAGGGCGGGAAGCCGGACGTGGTGCAGATCGTCCGGAACTA
CAACGCCTACCTGCGCGCCAGCGACGACCTGCCTAAGATGTTCATCGAGTCCGACCCCGG
CTTCTTCAGCAACGCTATCGTGGAGGGCGCCAAGAAGTTCCCCAACACCGAGTTCGTGAA
GGTGAAGGGCCTCCACTTCTCCCAAGAGGACGCCCCTGATGAGATGGGGAAGTACATCAA
GAGCTTCGTCGAGCGCGTCCTCAAGAACGAGCAGAATTCTCACGGCTTCCCTCCCGAGGT
GGAGGAGCAGGCCGCCGGCACCCTGCCCATGAGCTGCGCCCAGGAGAGCGGCATGGATAG
ACACCCTGCTGCTTGCGCCAGCGCCAGGATCAACGTCTAATCTAGAGTCGGGGCGGCCGG
CCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATG
CAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATT
ATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAG
GGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAT
AAGGATCCGTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGC
GCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGG
ACAGGTGCCGGCAGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC
GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAG
GGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAA
AGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATC
GACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCC
CTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTT
CGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACC
GCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGC
CACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAG
AGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAA
CCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAG
GATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACT
CACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAA
ATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCG
GCCGCAAATGCTAAACCACTGCAGTGGTTACCAATGCTTAATCAGTGAGGCACCTATCTC
AGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTAC
GATACGGGAGGGCTTACCATCTGGCCCCAGCGCTGCGATGATACCGCGAGAACCACGCTC
ACCGGCTCCGGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGG
TCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAG
TAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATCGCTACAGGCATCGTGGTGTC
ACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTAC
ATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAG
AAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTAC
TGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTG
AGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGC
GCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACT
CTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTG
ATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAA
TGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCGTCCTTTT
TCAATATTATTGAAGCATTTATCAGGGTTACTAGTACGTCTCTCAAGGATAAGTAAGTAA
TATTAAGGTACGGGAGGTATTGGACAGGCCGCAATAAAATATCTTTATTTTCATTACATC
TGTGTGTTGGTTTTTTGTGTGAATCGATAGTACTAACATACGCTCTCCATCAAAACAAAA
CGAAACAAAACAAACTAGCAAAATAGGCTGTCCCCAGTGCAAGTGCAGGTGCCAGAACAT
TTCTCT