Active Motif,
Tools to analyze nuclear function,
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Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3´UTR-reporter constructs and a microRNA mimic in adherent cells

Video: Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3´UTR-reporter constructs and a microRNA mimic in adherent cells

Here we describe a method using the LightSwitch Luciferase Assay System for the co-transfection of individual 3'UTR luciferase reporter constructs with a miRNA mimic. This protocol is efficient, reproducible, and amenable to high-throughput analysis.

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