Chromeo™ 494 Goat anti-Mouse IgG

Catalog No: 15032 Format: 1 mg $185 Buy
Catalog No: 15052 Format: 100 µg $105 Buy
Application:Immunofluorescence
Absorption:489 nm
Emission:624 nm
Molar Extinction:55,000 M-1 cm-1
Quantum Yield:~7%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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Contents

Contents: 1 mg (Catalog No. 15032) or 0.1 mg (Catalog No. 15052) of Chromeo™ 494 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo 494 is a unique dye with special spectral properties. It is excitable with a green laser and has a large Stokes shift of 134 nm. Chromeo 494 conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 494 and 628 nm, making them ideal partners for other 488 nm excitable dyes and conjugates.

Application Notes

Applications:
Immunofluorescence: 1:100 to 1:250 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

To ensure a maximum in photostability of Chromeo 494 conjugates under all experimental conditions, we recommend the use of Mowiol or TDE as mounting medium. Vectashield or MAXfluor Mounting medium might negatively influence the photostability of Chromeo 494.

Chromeo 494 Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leica's STimulated Emission Depletion (STED) microscopes. STED enables resolution of 50-70 nm.

 
Absorption and emission spectra of Chromeo 494 Dye.

Absorption and emission spectra of Chromeo 494 Dye.

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.

Active Motif's primary antibodies and fluorescent secondary antibodies in STED microscopy.
HeLa cells were stained with alpha Tubulin mouse monoclonal antibody (Clone 5-B-1-2, Cat. No. 39527) and Chromeo 494 Goat anti-Mouse IgG. Histone H3 was stained with Histone H3 trimethyl Lys4 rabbit polyclonal antibody (Cat. No. 39159) and the ATTO 647N (STED/GSD) Goat anti-Rabbit IgG (Cat. No. 15048) secondary antibody. This STED (STimulated Emission Depletion) image is courtesy of Leica Microsystems, Germany.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.