Chromeo™ 642 Goat anti-Mouse IgG

Catalog No: 15034 Format: 1 mg $185 Buy
Catalog No: 15054 Format: 100 µg $105 Buy
Application:Immunofluorescence
Absorption:647 nm
Emission:666 nm
Molar Extinction:180,000 M-1 cm-1
Quantum Yield:~7%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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Contents

Contents: 1 mg (Catalog No. 15034) or 0.1 mg (Catalog No. 15054) of Chromeo™ 642 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo 642 is spectrally similar to Cy5®. The conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo 642 conjugated antibodies have absorption and emission maxima of approximately 642 and 666 nm, which are compatible with common excitation sources and filter sets.

Application Notes

Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

 
Absorption and emission spectra of Chromeo 642 Dye.

Absorption and emission spectra of Chromeo 642 Dye.

Chromeo 642 staining in HeLa cells.

Chromeo 642 staining in HeLa cells.
HeLa cells were stained with alpha Tubulin mouse mAb (Clone 5-B-1-2; Cat. No. 39527) and Chromeo 642 Goat anti-mouse IgG. The nuclei have been counterstained with DAPI.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.