Chromeo™ 546 Goat anti-Rabbit IgG

Catalog No: 15043 Format: 1 mg $185 Buy
Catalog No: 15063 Format: 100 µg $105 Buy
Application:Immunofluorescence
Absorption:550 nm
Emission:567 nm
Molar Extinction:96,800 M-1 cm-1
Quantum Yield:~11%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

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Contents

Contents: 1 mg (Catalog No. 15043) or 0.1 mg (Catalog No. 15063) of Chromeo™ 546 conjugated Goat anti-Rabbit IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo 546 is spectrally similar to Cy3®. The conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm, which are compatible with common excitation sources and filter sets.

Application Notes

Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

Chromeo 546 Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of Chromeo 546 Dye.

Absorption and emission spectra of Chromeo 546 Dye.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.