Chromeo™ 642 Goat anti-Rabbit IgG

Catalog No: 15044 Format: 1 mg $185 Buy
Catalog No: 15064 Format: 100 µg $105 Buy
Application:Immunofluorescence
Absorption:647 nm
Emission:666 nm
Molar Extinction:180,000 M-1 cm-1
Quantum Yield:~7%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

Request a quote for a bulk orderBulk Order Button


Contents

Contents: 1 mg (Catalog No. 15044) or 0.1 mg (Catalog No. 15064) of Chromeo™ 642 conjugated Goat anti-Rabbit IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.

Chromeo™ 642 is spectrally similar to Cy5®. The conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. The Chromeo™ 642 conjugated antibodies have absorption and emission maxima of approximately 642 and 666 nm which are compatible with common excitation sources and filter sets.

Application Notes

Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution

 
Absorption and emission spectra of Chromeo 642 Dye.

Absorption and emission spectra of Chromeo 642 Dye.

Chromeo 642 staining of etoposide-treated HeLa cells.

Chromeo 642 staining of etoposide-treated HeLa cells.
HeLa cells were treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG.

Concurrent, multi-color staining of Phospho H2AX and tubulin using Chromeo 488 and Chromeo 494 secondary antibodies in untreated and etoposide-treated HeLa cells.

Concurrent, multi-color staining of Phospho H2AX and tubulin using Chromeo 642 and Chromeo 488 secondary antibodies in untreated and etoposide-treated HeLa cells.
HeLa cells were either left untreated or treated with 100 µM etoposide for 6 hours prior to fixation with methanol. The histone-variant H2AX was stained with Histone H2AX phospho Ser139 rabbit pAb (Cat. No. 39117) and Chromeo 642 Goat anti-rabbit IgG, while tubulin was visualized using alpha Tubulin mouse mAb (Clone 5-B-1-2) and Chromeo 488 Goat anti-mouse IgG (Cat. No. 15031). The nuclei were counter-stained with DAPI, then the three separate images were merged.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.