ATTO 655 (STED) Goat anti-Rabbit IgG

Catalog No: 15049 Format: 250 µl $310 Buy
Catalog No: 15069 Format: 35 µl $130 Buy
Application:Immunofluorescence
Absorption:663 nm
Emission:684 nm
Molar Extinction:125,000 M-1 cm-1
Quantum Yield:~30%
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

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Contents

Contents: 250 µl (Catalog No. 15049) or 35 µl (Catalog No. 15069) of affinity purified goat anti-rabbit IgG (H+L) conjugated to ATTO 655 (STED) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule rabbit IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, mouse, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

Characteristic features of the ATTO 655 (STED) are strong absorption, good fluorescence quantum yield, excellent thermal and photo stability, outstanding ozone resistance, very good water solubility, and very little triplet formation.

Application Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

ATTO 655 (STED) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using Leica's STimulated Emission Depletion (STED) microscopes. STED enables resolution of 50-70 nm.

 
Absorption and emission spectra of ATTO 655 (STED) Dye.

Absorption and emission spectra of ATTO 655 (STED) Dye.

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Rabbit IgG secondary antibody

Immunofluorescence staining using the ATTO 655 (STED) Goat anti-Rabbit IgG secondary antibody.
HeLa cells stained with Histone H3 trimethyl Lys27 rabbit polyclonal antibody (Cat. No. 39155) at 1:500 dilution and ATTO 647N (STED) Goat anti-Rabbit IgG secondary antibody at 1:1,000. Green: Nucleolin mouse monoclonal antibody (Cat. No. 39541).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.