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ATTO 532 (GSD) Goat anti-Rabbit IgG
| Catalog No: 15072 | Format: 250 µl | $305 | Buy Now |
| Catalog No: 15073 | Format: 35 µl | $125 | Buy Now |
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for ATTO 532 (GSD) Goat anti-Rabbit IgGContents
Contents: 250 µl (Catalog No. 15072) or 35 µl (Catalog No. 15073) of affinity purified goat anti-rabbit IgG (H+L) conjugated to ATTO 532 (GSD) in PBS, pH 7.4, 0.01% sodium azide.
Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule rabbit IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, mouse, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.
ATTO 532 (GSD) belongs to a class of fluorescent labels for the orange-red spectral region, like Rhodamine 6G. The dye is very photostable, exhibits a strong absorption and an excellent fluorescence quantum yield. ATTO 532 (GSD) is very soluble in water, allowing conjugations with high dye-to-protein ratios.
for ATTO 532 (GSD) Goat anti-Rabbit IgGApplication Notes
Application:
Immunofluorescence: 1:1,000 to 1:2,000
ATTO 532 (GSD) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.
Absorption and emission spectra of ATTO 532 (GSD) Dye.
ATTO 532 (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.
Tubulin was stained with a primary polyclonal rabbit antibody and with ATTO 532 (GSD) Goat anti-Rabbit IgG, then imaged using widefield (left) and GSDIM (right) microscopes. GSDIM provides much sharper resolution. These images are courtesy of Leica Microsystems, Germany.
for ATTO 532 (GSD) Goat anti-Rabbit IgGStorage
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for ATTO 532 (GSD) Goat anti-Rabbit IgGGuarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
