ATTO 532 (GSD) Goat anti-Rabbit IgG

Catalog No: 15072 Format: 250 µl $310 Buy
Catalog No: 15073 Format: 35 µl $130 Buy
Application:Immunofluorescence
Absorption:534 nm
Emission:560 nm
Molar Extinction:115,000 M-1 cm-1
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Rabbit
Purification:Affinity Purified

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Contents

Contents: 250 µl (Catalog No. 15072) or 35 µl (Catalog No. 15073) of affinity purified goat anti-rabbit IgG (H+L) conjugated to ATTO 532 (GSD) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule rabbit IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, mouse, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

ATTO 532 (GSD) belongs to a class of fluorescent labels for the orange-red spectral region, like Rhodamine 6G. The dye is very photostable, exhibits a strong absorption and an excellent fluorescence quantum yield. ATTO 532 (GSD) is very soluble in water, allowing conjugations with high dye-to-protein ratios.

Application Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

ATTO 532 (GSD) Goat anti-Rabbit IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of ATTO 532 (GSD) Dye.

Absorption and emission spectra of ATTO 532 (GSD) Dye.

ATTO 532 (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.

ATTO 532 (GSD) Goat anti-Rabbit secondary antibody conjugate in widefield and GSDIM microscopy.
Tubulin was stained with a primary polyclonal rabbit antibody and with ATTO 532 (GSD) Goat anti-Rabbit IgG, then imaged using widefield (left) and GSDIM (right) microscopes. GSDIM provides much sharper resolution. These images are courtesy of Leica Microsystems, Germany.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.