Rhodamine 6G (GSD) Goat anti-Mouse IgG

Catalog No: 15074 Format: 250 µl $310 Buy
Catalog No: 15075 Format: 35 µl $130 Buy
Application:Immunofluorescence
Absorption:508 nm
Emission:558 nm
Molar Extinction:116,000 M-1 cm-1
Antibody Type:Polyclonal
Isotype:IgG
Host:Goat
Reactivity:Mouse
Purification:Affinity Purified

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Contents

Contents: 250 µl (Catalog No. 15074) or 35 µl (Catalog No. 15075 of affinity purified goat anti-mouse IgG (H+L) conjugated to Rhodamine 6G (GSD) in PBS, pH 7.4, 0.01% sodium azide.

Specificity: This antibody was purified by immunoaffinity chromatography and cross-adsorbed. It reacts with whole molecule mouse IgG and shows minimum cross-reactivity with bovine, chicken, goat, guinea pig, horse, human, rabbit, rat and sheep serum proteins. No cross-reactivity was observed with non-immunoglobulin serum proteins.

Rhodamine 6G (GSD) belongs to a class of fluorescent labels for the orange-red spectral region. Characteristic features of the label are strong absorption, excellent fluorescence quantum yield, and high photo stability.

Application Notes

Application:
Immunofluorescence: 1:1,000 to 1:2,000

The Rhodamine 6G (GSD) Goat anti-Mouse IgG is recommended by Leica Microsystems for Super-Resolution Fluorescence Microscopy using the Leica SR GSD microscope for performing Ground State Depletion with Individual Molecule return (GSDIM) microscopy. GSDIM can mathematically re-construct images with resolution as low as 20 nm.

 
Absorption and emission spectra of Rhodamine 6G (GSD) Dye.

Absorption and emission spectra of Rhodamine 6G (GSD) Dye.

Rhodamine 6G (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.

Rhodamine 6G (GSD) Goat anti-Mouse secondary antibody conjugate in widefield and GSDIM microscopy.
Tubulin was stained with a primary monoclonal mouse antibody and with Rhodamine 6G (GSD) Goat anti-Mouse IgG, then imaged using widefield (left) and GSDIM (right) microscopes. GSDIM provides much sharper resolution. These images are courtesy of Leica Microsystems, Germany.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.