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Purified Sp1 protein

Catalog No: 31137 Format: 2 µg
Expressed In: HeLa cells Protein Species: Human

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for Purified Sp1 proteinContents

This product has been discontinued

2 µg of Purified Sp1 protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA).

for Purified Sp1 proteinBackground

Sp1 (specificity protein 1) is a human transcription factor involved in gene expression in the early development of an organism. The protein is 785 amino acids long, with a molecular weight of 81 kDa. The SP1 transcription factor contains a zinc finger protein motif, by which it binds directly to DNA and enhances gene transcription. Its zinc fingers are of the Cys2/His2 type and bind the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ (GC box element). It was discovered in 1983 and has since been modified to form Sp1C, which has a zinc finger protein DNA binding domain.

for Purified Sp1 proteinApplication Notes

Purified Sp1 is suitable for in vitro transcription, DNase I protection and gelshift assays. 50-100 ng is sufficient for reconstituted transcription assays and 5-25 ng in a 20 µl volume are sufficient for gelshift assays. The molecular weight of the protein is ~105 kDa. This protein is not recommended for TransAM® assays. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.

for Purified Sp1 proteinProtein Details

The natural Sp1 fraction (accession number NM 138473) was purified from HeLa cell nuclear extract by using wheat germ agglutinin affinity chromatography. The purified protein is greater than 95% homogeneous and contains no detectable protease, DNase or RNase activity.

for Purified Sp1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Purified Sp1 protein