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Recombinant Proteins & Enzymes

Recombinant PKCα protein

Aliases: AAG6, PRKCA, PRKACA, PKC-alpha
Catalog No: 31159 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant PKCa proteinContents

This product has been discontinued

10 µg of Recombinant PKCα protein in 20 mM NaH2PO4, 500 mM NaCl, 1 mM sodium orthovanadate, 5 mM NaF, 40 mM beta-glycerophosphate, 5% glycerol, pH 7.8.

for Recombinant PKCa proteinApplication Notes

Recombinant PKCα is suitable for kinase assays. The molecular weight of the protein is ~81.912 kDa. The activity of the protein is ~ 355,000 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to PKC peptide (QKRPSQRSKYL) per minute at 30°C. Recommended Kinase Reaction Buffer: 20 mM Tris-HCL, 25 mM β-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate and 1 mM DTT, pH 7.5. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant PKCa proteinProtein Details

Recombinant human PKCα protein was expressed in Sf9 cells with a C-terminal His-Tag and is suitable for labeling PKC substrates. The accession number is X52479. Purified by Ni-agarose chromatography to > 95% by SDS-PAGE.

for Recombinant PKCa proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant PKCα protein