Recombinant GSK3β protein

Aliases: Glycogen Synthase Kinase 3 Beta, GSK-3 Beta
Catalog No: 31173 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

Request a quote for a bulk orderBulk Order Button


Contents


This product has been discontinued:
Please refer to our alternative product Catalog No. 81194

10 µg of Recombinant GSK3β protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

Application Notes

Recombinant GSK3β is suitable for kinase assays and Western blot. The molecular weight of the protein is ~76.312 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 5 µg/50 µl, Recombinant GSK3β: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

Protein Details

Recombinant human GSK3β protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-T420 , accession number L33801 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

 
Recombinant GSK3β protein, active analyzed by SDS-PAGE gel.

Recombinant GSK3β protein tested by SDS-PAGE gel.
0.5 µg of GSK3β protein was analyzed using SDS/PAGE followed by Western blot with either an anti-GSK3β or anti-GST antibody.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.