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Recombinant Proteins & Enzymes

Recombinant JNK3 protein

Aliases: Mitogen-Activated Protein Kinase 10, MAPK10, JNK3A, PRKM10, SAPK1b, p493F12, p54bSAPK
Catalog No: 31183 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant JNK3 proteinContents

This product has been discontinued

10 µg of Recombinant JNK3 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

for Recombinant JNK3 proteinApplication Notes

Recombinant JNK3 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~82.161 kDa. The activity of the protein is ~ 0.2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF 2 µg / 50 µl, Recombinant JNK3 : 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant JNK3 proteinProtein Details

Recombinant human JNK3 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-Q422, accession number NM_002753 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

Recombinant JNK3 protein, active analyzed by SDS-PAGE gel.

Recombinant JNK3 protein tested by SDS-PAGE gel.
0.5 µg of JNK3 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-JNK3 or anti-GST antibody.

for Recombinant JNK3 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Recombinant JNK3 protein

Data Thumbnails

Recombinant JNK3 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant JNK3 protein, active.
(Click image to enlarge and see details.)