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Recombinant S6K protein

Aliases: RPS6KB1, PS6K, S6K1, STK14A, p70-S6K, p70 S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha
Catalog No: 31193 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant S6K proteinContents

This product has been discontinued

10 µg of Recombinant S6K protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

for Recombinant S6K proteinApplication Notes

Recombinant S6K is suitable for kinase assays and Western blot. The molecular weight of the protein is ~74.253 kDa. The activity of the protein is ~ 13 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: R11-S6-Peptide (R11-IAKRRRLSSLRASTSKSESSQK), 5 µg / 50 µl, Recombinant S6K: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant S6K proteinProtein Details

Recombinant human S6K protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-K400, accession number M60725 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.

Recombinant S6K protein, active analyzed by SDS-PAGE gel.

Recombinant S6K protein tested by SDS-PAGE gel.
0.5 µg of S6K protein was analyzed using SDS/PAGE followed by Western blot with either an anti-S6K or anti-GST antibody.

for Recombinant S6K proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant S6K protein

Data Thumbnails

Recombinant S6K protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant S6K protein, active.
(Click image to enlarge and see details.)