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Recombinant SNK protein

Aliases: PLK2, hSNK, hPlk2
Catalog No: 31194 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant SNK proteinContents

This product has been discontinued

10 µg of Recombinant SNK protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.

for Recombinant SNK proteinApplication Notes

Recombinant SNK is suitable for kinase assays and Western blot. The molecular weight of the protein is ~107.435 kDa. The activity of the protein is ~ 2 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: Rb-CTF, 10 µg / 50 µl, Recombinant SNK: 200 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant SNK proteinProtein Details

Recombinant human SNK protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-N685, accession number NM_006622 and N-terminally fused to GST-HIS6-Thrombin site. Purified by GSH-agarose affinity purification.

Recombinant SNK protein, active analyzed by SDS-PAGE gel.

Recombinant SNK protein tested by SDS-PAGE gel.
0.5 µg of SNK protein was analyzed using SDS/PAGE followed by Western blot with either an anti-GSK or anti-SNK antibody.

for Recombinant SNK proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant SNK protein

Data Thumbnails

Recombinant SNK protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant SNK protein, active.
(Click image to enlarge and see details.)