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Recombinant Proteins & Enzymes

Recombinant VEGF-R1 protein

Aliases: FLT, FLT-1, VEGFR1, VEGFR-1, FLT1
Catalog No: 31197 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant VEGF-R1 proteinContents

This product has been discontinued

10 µg of Recombinant VEGF-R1 protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 15 mM reduced glutathione, 20% glycerol.

for Recombinant VEGF-R1 proteinApplication Notes

Recombinant VEGF-R1 is suitable for kinase assays and Western blot. The molecular weight of the protein is ~89.357 kDa. The activity of the protein is ~ 53 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 1.5 µg / 50 µl, Recombinant VEGF-R1: 100 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant VEGF-R1 proteinProtein Details

Recombinant human VEGF-R1 protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids K784-I1338, accession number NM_002019 and N-terminally fused to GST-Factor Xa cleavage site. Purified by GSH-agarose affinity purification.

Recombinant VEGF-R1 protein, active analyzed by SDS-PAGE gel.

Recombinant VEGF-R1 protein tested by SDS-PAGE gel.
0.5 µg of VEGF-R1 protein was analyzed using SDS/PAGE followed by Western blot with either an anti-VEGF-RI or anti-GST antibody.

for Recombinant VEGF-R1 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant VEGF-R1 protein

Data Thumbnails

Recombinant VEGF-R1 protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant VEGF-R1 protein, active.
(Click image to enlarge and see details.)