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Recombinant Yes protein

Aliases: YES1; c-yes; HsT441; P61-YES
Catalog No: 31202 Format: 10 µg
Expressed In: Baculovirus Protein Species: Human

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for Recombinant Yes proteinContents

This product has been discontinued

10 µg of Recombinant Yes protein in 50 mM Tris-HCl, pH 8.0; 100 mM NaCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol.

for Recombinant Yes proteinApplication Notes

Recombinant Yes is suitable for kinase assays and Western blot. The molecular weight of the protein is ~90.997 kDa. The activity of the protein is ~ 29 pmol/µg min. Recommended kinase reaction conditions: 60 mM HEPES-NaOH, pH 7.5, 3 mM MgCl2, 3 mM MnCl2, 3 µM Na-orthovanadate, 1.2 mM DTT, ATP (variable), 2.5 µg/50 µl PEG20.000, Substrate: PolyEY, 1 µg / 50 µl, Recombinant Yes: 50 ng/50 µl. Kinase activity may vary depending on the substrate and reaction conditions used.

for Recombinant Yes proteinProtein Details

Recombinant human Yes protein was produced using baculovirus infected Sf9 cells. The protein was made against amino acids M1-L543, accession number NM_005433 and N-terminally fused to a GST-HIS6-Thrombin cleavage site. Purified by GSH-agarose affinity purification.

Recombinant Yes protein, active analyzed by SDS-PAGE gel.

Recombinant Yes protein tested by SDS-PAGE gel.
0.5 µg of Yes protein was analyzed using SDS/PAGE followed by Western blot with either an anti-Yes or anti-GST antibody.

for Recombinant Yes proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Technical Data Sheet

Recombinant Yes protein

Data Thumbnails

Recombinant Yes protein, active analyzed by SDS-PAGE gel.

SDS-PAGE gel of Recombinant Yes protein, active.
(Click image to enlarge and see details.)