Recombinant Histone H4 (Xenopus)

Catalog No: 31223 Format: 50 µg $395 Buy
Expressed In: E. coli Protein Species: Xenopus

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Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.

Background

Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Protein Details

Recombinant Histone H4 (Xenopus) is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for Histone H4 and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the recombinant histone is 11,211 Daltons.

References


This product was used in the following publications:
Audrey, J., et. al. (2018). "The inhibition of checkpoint activation by telomeres does not involve exclusion of dimethylation of histone H4 lysine 20 (H4K20me2)."F1000Res. July 9:7: 1027. PMID: 30498568. (Western Blot)

Li, J., et. al. (2018). "Nuclear PKC-theta facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation." J. Cell Sci. 129(12): 2448-61. PMID: 27149922. (in vitro kinase assay)

 

Recombinant Histone H4 (Xenopus) analyzed by SDS-PAGE gel.
SDS-PAGE analysis of 1.5 µg Recombinant Histone H4 (lane 2), Recombinant Histone H4 monomethyl Lys20 (lane 3), Recombinant Histone H4 dimethyl Lys20 (lane 4), and Recombinant Histone H4 trimethyl Lys20 (lane 5). Molecular weight marker is in lane 1.

Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.