Recombinant Histone H2A (Xenopus)

Catalog No: 31251 Format: 50 µg $395 Buy
Expressed In: E. coli Protein Species: Xenopus

Contents

A representative Technical Data Sheet (TDS) is provided here. Please refer to your lot-specific TDS upon arrival for specific buffer contents and lot-specific protein concentration.

Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.

Application Notes

Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.

Protein Details

Recombinant Histone H2A (Xenopus) is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for histone H2A and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the protein is 13,950 Daltons.

 

Recombinant Histone H2A (Xenopus) analyzed by SDS-PAGE gel.
5 µg, 2 µg and 1 µg respectively of Recombinant Histone H2B, Catalog No. 31252 (lanes 1-3), Recombinant Histone H2A, Catalog No. 31251 (lanes 4-6) and Recombinant Histone H3, Catalog No. 31207 (lanes 7-9)

Storage

Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.