Expressed In: E. coli Protein Species: Xenopus
A representative Technical Data Sheet (TDS) is provided here. Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration.
Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
This product has been discontinued:
Please refer to our alternative products Catalog No. 31492
Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
Recombinant Histone H2B (Xenopus) is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for Histone H2B and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the protein is 13,494 Daltons. The H2B protein is missing the first three residues (PEP).
The protein sequence is as follows: AKSAPA PKKGSKKAVT KTQKKDGKKR RKSRKESYAI YVYKVLKQVH PDTGISSKAM SIMNSFVNDV FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSAK
This product was used in the following publications:
Li, J., et. al. (2016). "Nuclear PKC-theta facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation." J. Cell Sci. 129(12):2448-61. PMID: 27149922.
(in vitro kinase assay)
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.