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Recombinant Proteins & Enzymes

Recombinant UBCH6 protein

Aliases: UBE2E1
Catalog No: 31362 Format: 100 µg
Expressed In: E. coli Protein Species: Human

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for Recombinant UBCH6 proteinContents

This product has been discontinued

50 µg recombinant UbcH6 expressed in E. coli and supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT.

for Recombinant UBCH6 proteinBackground

UBCH6 (Ubiquitin-Conjugating Enzyme E2E 1) Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes Lys-48-linked polyubiquitination.

for Recombinant UBCH6 proteinApplication Notes

Recombinant UbcH6 is suitable for use in ubiquitin conjugating assays. A good starting point is 25 to 100 ng of enzyme per assay.

for Recombinant UBCH6 proteinProtein Details

Amino acids 2-193 of UBCH6 (accession number NP_003332.1) were expressed in E. coli with an N-terminal His-Tag (MW = 22 kDa).

UBCH6 protein Coomassie gel

UBCH6 protein gel.
UBCH6 run on an SDS-PAGE gel and stained with Coomassie blue.

UBCH6 activity assay

UBCH6 activity assay.
Recombinant UbcH6 activity measured using a luminescent ubiquitin conjugating assay.

for Recombinant UBCH6 proteinStorage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

for Recombinant UBCH6 proteinGuarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.


Technical Data Sheet

Recombinant UBCH6 protein

Data Thumbnails

UBCH6 protein Coomassie gel

UBCH6 protein Coomassie gel.
(Click image to enlarge and see details).

UBCH6 activity assay

UBCH6 activity assay.
(Click image to enlarge and see details).