Recombinant NSD2 (MMSET)-SET protein

Aliases: Nuclear Receptor Binding SET Domain Protein 2, WHS, NSD2, TRX5, REIIBP
Catalog No: 31476 Format: 50 µg $410 Buy
Catalog No: 31776 Format: 1 mg $3,500 Buy
Expressed In: E. coli Protein Species: Human

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Recombinant NSD2 (MMSET)-SET (aa 942-1240) is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100.


NSD2 (MMSET)-SET (Multiple Myeloma SET domain-containing protein, WHSC1/Wolf-Hirschhorn Syndrome Candidate 1, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The NSD2 (MMSET)-SET gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to NSD2 (MMSET)-SET gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in NSD2 (MMSET)-SET expression in a myeloma cell line suppressed its growth, suggesting a role for NSD2 (MMSET)-SET in maintaining the transformed state.

NSD2 (MMSET)-SET includes the SET domain of NSD2 (MMSET)-SET. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity.

Application Notes

Recombinant NSD2 (MMSET)-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 0.6 µM recombinant nucleosomes were incubated with 0.1 µM recombinant NSD2 (MMSET)-SET protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hours at room temperature. Activity was detected by Western Blot.

Protein Details

Recombinant NSD2 (MMSET)-SET peptide that includes amino acids 942-1240 that contains the SET domain sequence of the human NSD2 (MMSET)-SET protein (accession number NP_579877.1) was expressed in E. coli cells and contains an N-terminal GST-Tag with an observed molecular weight of 60 kDa. The recombinant protein is >90% pure by SDS-PAGE.

Recombinant NSD2 (MMSET)-SET protein gel.

Recombinant NSD2 (MMSET)-SET protein gel.
Recombinant NSD2 (MMSET)-SET run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant NSD2 (MMSET)-SET activity assay.

Recombinant NSD2 (MMSET)-SET activity assay.
2 µg recombinant nucleosomes (Cat. No. 31468) were incubated with NSD2 (MMSET)-SET protein in reaction buffer for 3 hours at room temperature. Western blot was used to detect the generation of reaction products (H3K36me2, Cat. No. 39891). NSD2 (MMSET)-SET only was used as negative control.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.