Recombinant NSD2 (MMSET)-SET protein

Aliases: Nuclear Receptor Binding SET Domain Protein 2, WHS, NSD2, TRX5, REIIBP
Catalog No: 31476 Format: 50 µg $410 Buy
Catalog No: 31776 Format: 1 mg $3,500 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant NSD2 (MMSET)-SET (aa 942-1240) is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100.

Background

NSD2 (MMSET)-SET (Multiple Myeloma SET domain-containing protein, WHSC1/Wolf-Hirschhorn Syndrome Candidate 1, Nuclear Receptor binding SET domain protein 2, TRX5, Trithorax 5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The NSD2 (MMSET)-SET gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to NSD2 (MMSET)-SET gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in NSD2 (MMSET)-SET expression in a myeloma cell line suppressed its growth, suggesting a role for NSD2 (MMSET)-SET in maintaining the transformed state.


NSD2 (MMSET)-SET includes the SET domain of NSD2 (MMSET)-SET. The SET domain is a 130 to 140 amino acid, evolutionary conserved motif that was initially characterized in the Drosophila proteins Su(var)3-9, EZH2 and TrxG and plays a crucial role in substrate recognition and enzymatic activity.

Application Notes

Recombinant NSD2 (MMSET)-SET protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 0.6 µM recombinant nucleosomes were incubated with 0.1 µM recombinant NSD2 (MMSET)-SET protein in reaction buffer including 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM for 3 hours at room temperature. Activity was detected by Western Blot.

Protein Details

Recombinant NSD2 (MMSET)-SET peptide that includes amino acids 942-1240 that contains the SET domain sequence of the human NSD2 (MMSET)-SET protein (accession number NP_579877.1) was expressed in E. coli cells and contains an N-terminal GST-Tag with an observed molecular weight of 60 kDa. The recombinant protein is >90% pure by SDS-PAGE.

 
Recombinant NSD2 (MMSET)-SET protein gel.

Recombinant NSD2 (MMSET)-SET protein gel.
Recombinant NSD2 (MMSET)-SET run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Recombinant NSD2 (MMSET)-SET activity assay.

Recombinant NSD2 (MMSET)-SET activity assay.
2 µg recombinant nucleosomes (Cat. No. 31468) were incubated with NSD2 (MMSET)-SET protein in reaction buffer for 3 hours at room temperature. Western blot was used to detect the generation of reaction products (H3K36me2, Cat. No. 39891). NSD2 (MMSET)-SET only was used as negative control.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.