Recombinant LSD1 / KDM1A protein

Aliases: Lysine Demethylase 1A, Flavin-Containing Amine Oxidase Domain-Containing Protein 2, Lysine-Specific Histone Demethylase 1
Catalog No: 31507 Format: 50 µg
Expressed In: E. coli Protein Species: Human

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Contents


This product has been discontinued:
Please refer to our alternative product Catalog No. 31426

Recombinant LSD1 / KDM1A protein expressed in E. coli cells and supplied in 20 mM Na2HPO4/ NaH2PO4, pH 7.4, 300 mM NaCl, 16 mM glutathione, 1 mM EDTA, 0.05%Tween-20, and 20% glycerol.

Background

LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase.

Application Notes

Recombinant LSD1 / KDM1A is suitable for use in histone demethylation assays. A recommended starting point for the demethylation assay is 20-100 ng of recombinant protein per reaction.

Protein Details

LSD1 (KDM1A, AOF2) is a histone demethylase that is specific to histone H3 dimethylated at lysine 4 (H3K4me2). Methylation of H3K4 is a marker of transcriptional activation. Recombinant full-length human LSD1 (GenBank Accession No. NM_015013), amino acids 158-end with N-terminal GST-Tag, was expressed in E. coli. The molecular weight of the protein is 103 kDa. The recombinant protein is >70% pure by SDS-PAGE.

 
LSD1 / KDM1A Coomassie gel

Recombinant LSD1 / KDM1A protein gel.
LSD1 / KDM1A run on a 4-20% SDS-PAGE gel and stained with Coomassie blue.

LSD1 / KDM1A activity assay

Recombinant LSD1 / KDM1A protein activity assay.
Recombinant LSD1 / KDM1A activity measured using a fluorescent demethylase assay.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.