Recombinant IRF3 protein

Aliases: Interferon Regulatory Factor 3, IRF-3
Catalog No: 31544 Format: 20 µg $410 Buy
Expressed In: Baculovirus Protein Species: Human

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Contents

Recombinant full length IRF3 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. Please refer to product insert upon arrival for lot-specific concentration.

Background

IRF3 (Interferon regulatory factor 3)is a member of the IRF family of transcription factors that include IRF1, IRF6 and IRF7. IRF3 that has been phosphorylated at serine/threonine forms a complex with CREBBP and translocates to the nucleus to activate transcription of interferon-induced genes. IRF3 plays a critical role in the innate immune response against DNA and RNA viruses.

Application Notes

Recombinant IRF3 Protein is useful for the study of protein-protein interaction, screening inhibitors, and selectivity profiling. This product was manufactured as described in Protein Details. Where possible, Active Motif has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.

Protein Details

Recombinant human IRF3 was expressed in a baculovirus expression system as the full length protein (accession number NP_001562.1) with an N-terminal FLAG-Tag. The molecular weight of the protein is of 50.2 kDa. The IRF3 protein is >90% pure by SDS-PAGE.

 
Recombinant IRF3 protein gel.

Recombinant IRF3 protein gel.
IRF3 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.