Recombinant BRPF1 (629-742), GST-Tag

Aliases: Bromodomain And PHD Finger Containing 1, Peregrin, BR140
Catalog No: 31547 Format: 100 µg $410 Buy
Catalog No: 31947 Format: 1 mg $2,900 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant BRPF1 (629-742), GST-Tag is suppled in 25 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

Bromodomain and PHD  finger containing (BRPF1) protein is a component of the MOZ/MORF complex which has histone H3 acetyltransferase activity. The bromodomain of BRPF1 recognizes acetylated histone lysine residues and function as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BRPF1 is known to positively regulate the transcription of RUNX1 and RUNX2.

Application Notes

Recombinant BRPF1 (629-742), GST-Tag is suitable for use in binding assays, inhibitor screening, and selectivity profiling. 

Protein Details

The peptide corresponding to amino acids 629 - 742 that contains the bromodomain sequences of BRPF1 (accession number NP_004625.2) was expressed in E. coli cells and contains an N-terminal GST-Tag with a molecular weight of 39.4 kDa. BRPF1 (629-742), GST-Tag is >92% pure by SDS-PAGE.

 
Recombinant BRPF1 (629-742), GST-Tag gel.

Recombinant BRPF1 (629-742) Protein SDS-PAGE Gel.
BRPF1 (629-742) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue.

HTRF assay for BRPF1 (629-742) protein activity.

HTRF assay for BRPF1 (629-742) protein activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.

HTRF assay for BRPF1 (629-742), GST-Tag activity

HTRF assay for BRPF1 (629-742) protein activity.
3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BRPF1 (629-742), GST-Tag in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.