Recombinant NSD2-SET (E1099K) protein

Aliases: nuclear receptor binding SET domain protein 1, WHSC1, MMSET, TRX5
Catalog No: 31574 Format: 50 µg $410 Buy
Catalog No: 31974 Format: 1 mg $3,500 Buy
Expressed In: E. coli Protein Species: Human

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Contents

Recombinant NSD2-SET (E1099K) protein is suppled in 50 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.

Background

NSD2 (nuclear receptor binding SET domain protein 1; also known as WHSC1; MMSET; TRX5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The NSD2 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to NSD2 gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in NSD2 expression in a myeloma cell line suppressed its growth, suggesting a role for NSD2 in maintaining the transformed state.
NSD2 p.Glu1099Lys (p.E1099K) variant was identified in nontranslocated acute lymphoblastic leukemia (ALL) cell lines.

Application Notes

Recombinant NSD2-SET (E1099K) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Protein Function: H3K36me2 methyltransferase activity. HMT Assay Conditions: 60 ng/µl recombinant nucleosomes was incubated with 30 ng/µl NSD2-SET (E1099K) protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM, for 3 hours at room temperature. Activity was detected by Western Blot.

Protein Details

Recombinant human NSD2-SET (E1099K) that includes amino acids 942-1240 (accession number NP_579877.1) was expressed in E. coli cells with a point mutation (Glu1099Lys) and an N-terminal GST-Tag. The molecular weight of the protein is 60 kDa. NSD2-SET (E1099K) protein is >85% pure by SDS-PAGE.

 
Recombinant NSD2-SET (E1099K) protein gel.

Recombinant NSD2-SET (E1099K) protein gel.
NSD2-SET (E1099K) protein was run on a 12% SDS- PAGE gel and stained with Coomassie Blue. The purity of NSD2-SET (E1099K): ≥ 85%

Recombinant NSD2-SET (E1099K) activity assay.

Recombinant NSD2-SET (E1099K) activity assay.
2 µg recombinant nucleosomes (Cat #: 31468) were incubated with NSD2-SET (E1099K) in reaction buffer for 3 hours at room temperature. 2 µg histone octamer (Cat #: 31472) was used as a control substrate. Reaction product (H3K36me2) was detected by Western Blot.

Storage

Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Guarantee

This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.