Recombinant NSD2-SET (E1099K) protein

Aliases: nuclear receptor binding SET domain protein 1, WHSC1, MMSET, TRX5
Catalog No: 31574 Format: 50 µg $410 Buy
Catalog No: 31974 Format: 1 mg $3,500 Buy
Expressed In: E. coli Protein Species: Human

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Recombinant NSD2-SET (E1099K) protein is suppled in 50 mM Tris pH 8.0, 300 mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.


NSD2 (nuclear receptor binding SET domain protein 1; also known as WHSC1; MMSET; TRX5) is a SET domain-containing histone methyltransferase specific for lysine 36 of histone H3. The NSD2 gene is deleted in Wolf-Hirschhorn syndrome, a disorder characterized by developmental defects and mental retardation. In 15% of patients with Multiple Myeloma (MM), a chromosomal translocation leads to NSD2 gene being aberrantly placed under the control of the IgH intronic µ enhancer and expressed at very high levels. This event is apparently a key component in cellular transformation leading to MM. Reduction in NSD2 expression in a myeloma cell line suppressed its growth, suggesting a role for NSD2 in maintaining the transformed state.
NSD2 p.Glu1099Lys (p.E1099K) variant was identified in nontranslocated acute lymphoblastic leukemia (ALL) cell lines.

Application Notes

Recombinant NSD2-SET (E1099K) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Protein Function: H3K36me2 methyltransferase activity. HMT Assay Conditions: 60 ng/µl recombinant nucleosomes was incubated with 30 ng/µl NSD2-SET (E1099K) protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 µM SAM, for 3 hours at room temperature. Activity was detected by Western Blot.

Protein Details

Recombinant human NSD2-SET (E1099K) that includes amino acids 942-1240 (accession number NP_579877.1) was expressed in E. coli cells with a point mutation (Glu1099Lys) and an N-terminal GST-Tag. The molecular weight of the protein is 60 kDa. NSD2-SET (E1099K) protein is >85% pure by SDS-PAGE.

Recombinant NSD2-SET (E1099K) protein gel.

Recombinant NSD2-SET (E1099K) protein gel.
NSD2-SET (E1099K) protein was run on a 12% SDS- PAGE gel and stained with Coomassie Blue. The purity of NSD2-SET (E1099K): ≥ 85%

Recombinant NSD2-SET (E1099K) activity assay.

Recombinant NSD2-SET (E1099K) activity assay.
2 µg recombinant nucleosomes (Cat #: 31468) were incubated with NSD2-SET (E1099K) in reaction buffer for 3 hours at room temperature. 2 µg histone octamer (Cat #: 31472) was used as a control substrate. Reaction product (H3K36me2) was detected by Western Blot.


Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.


This product is for research use only and is not for use in diagnostic procedures. This product is guaranteed for 6 months from date of arrival.