JEG-3 nuclear extract

Catalog No: 36012 Format: 200 µg $185 Buy

Contents

200 µg of JEG-3 nuclear extract at 4.3 µg/µl.

Background

JEG-3 nuclear extract was prepared from cell cultures of the JEG-3 human placental choriocarcinoma cell line. These cells are epithelial in morphology and are derived from propagated extravillous trophoblast (EVT) cells of the placenta. EVT cells are a subpopulation of specialized and highly invasive cells. In response to a TGF-β signal secreted from the uterus, these cells are prompted to grow out from the placenta and invade the decidual lining of the uterus. Once they penetrate the decidua, they serve to both anchor the placenta to the uterus and establish an adequate blood supply exchange from the mother to the developing fetus. In research, JEG-3 cells commonly serve as trophoblastic model systems to study mechanisms regulating mammalian reproduction and fetal development. In addition, due to their invasive properties, these cells are also useful in dissecting the underlying processes governing proliferation, migration and invasiveness related to malignant cell behavior.

Application Notes

JEG-3 nuclear extract is specifically recommended for use in studies related to the mechanisms governing 1) proliferation, 2) migration, and 3) invasiveness during mammalian reproduction, fetal development and cancer.

Extract Origin

Human epithelial placenta; choriocarcinoma

Extract Composition

JEG-3 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.