T-47D nuclear extract

Catalog No: 36027 Format: 200 µg $175 Buy

Contents

200 µg of T-47D nuclear extract at 6.37 µg/µl.

Background

T-47D nuclear extract was prepared from cell cultures of the human ductal breast epithelial tumor cell line. The T-47D cell line was derived from a pleural effusion of a 54-year old female patient with infiltrating ductal carcinoma. These cells exhibit a differentiated epithelial cell phenotype and express receptors to estradiol, as well as other steroids, and calcitonin. These cells also express mutant tumor suppressor p53 and are therefore commonly used in research as in vitro models of mutant p53 breast cancer cells to study breast cancer tumorigenicity and related drug therapies, such as tamoxifen.

Application Notes

T-47D nuclear extract is specifically recommended for use in studies related to breast cancer and associated drug therapies.

Extract Origin

Human breast ductal carcinoma

Extract Composition

T-47D nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.