HT-29 nuclear extract

Catalog No: 36073 Format: 200 µg $175 Buy

Contents

200 µg of HT-29 nuclear extract at 5.6 µg/µl.

Background

HT-29 nuclear extract was prepared from a cell culture of the human colorectal adenocarcinoma HT-29 cell line. The HT-29 cell line was originally derived from intestinal tumor tissue obtained from a 44 year old Caucasian female patient with colorectal adenocarcinoma. HT-29 cells are morphologically epithelial, are CD4 negative, and produce carcinoembryonic antigen (CEA) and immunoglobulin A (IgA). This cell line contains a mutation in codon 273 of the p53 gene that results in an Arg>His substitution. In the absence of carbohydrates, HT-29 cells differentiate into polarized epithelium and, therefore, have been used as in vitro model systems to study epithelial differentiation. However, the HT-29 cell line is most commonly used in research as a model for colorectal cancer in studies related to growth and tumorigenicity.

Application Notes

HT-29 nuclear extract is recommended for use in studies related to colorectal cancer and epithelial differentiation.

Extract Origin

Human epithelial colon; colorectal adenocarcinoma

Extract Composition

HT-29 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Quality Control

Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

Storage

To ensure stability, extracts should be stored at -80°C.

We recommend aliquoting the extracts into single-use fractions and then storing them at -80°C. This eliminates repeated freeze/thaw cycles.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.